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Sputum stain for mycobacteria

Acid fast bacilli stain; AFB stain; Tuberculosis smear; TB smear

Sputum stain for mycobacteria is a test to check for a type of bacteria that cause tuberculosis and other infections.

How the Test is Performed

This test requires a sample of sputum. 

  • You will be asked to cough deeply and spit any substance that comes up from your lungs (sputum) into a special container.
  • You may be asked to breathe in a mist of salty steam. This makes you cough more deeply and produce sputum.
  • If you still do not produce enough sputum, you might have a procedure called bronchoscopy.
  • To increase the accuracy, this test is sometimes done three times, often 3 days in a row.

The test sample is examined under a microscope. Another test, called a culture, is done to confirm the results. A culture test takes a few days to get results. This sputum test can give your doctor a quick answer.

How to Prepare for the Test

Drinking a lot of fluids the night before the test helps your lungs produce phlegm. It makes the test more accurate if it is done first thing in the morning.

How the Test will Feel

There is no discomfort, unless a bronchoscopy needs to be performed.

Why the Test is Performed

The test is performed when the doctor suspects tuberculosis or other mycobacterium infection.

Normal Results

Results are normal when no mycobacterial organisms are found.

What Abnormal Results Mean

Abnormal results show that the stain is positive for:

  • Mycobacterium tuberculosis
  • Mycobacterium avium-intracellular
  • Other mycobacteria or acid-fast bacteria

Risks

There are no risks, unless bronchoscopy is performed.

References

Hopewell PC, Kato-Maeda M, Ernst JD. Tuberculosis. In: Broaddus VC, Mason RJ, Ernst JD, et al, eds. Murray and Nadel's Textbook of Respiratory Medicine. 6th ed. Philadelphia, PA: Elsevier Saunders; 2016:chap 35.

Woods GL. Mycobacteria. In: McPherson RA, Pincus MR, eds. Henry's Clinical Diagnosis and Management by Laboratory Methods. 23rd ed. St Louis, MO: Elsevier; 2017:chap 61.

    • Sputum test

      Sputum test - illustration

      A sputum sample is obtained by coughing deeply and expelling the material that comes from the lungs into a sterile cup. The sample is taken to a labarotory and placed in a medium under conditions that allow the organisms to grow. A positive culture may identify disease-producing organisms that may help diagnose bronchitis, tuberculosis, a lung abscess, or pneumonia.

      Sputum test

      illustration

      • Sputum test

        Sputum test - illustration

        A sputum sample is obtained by coughing deeply and expelling the material that comes from the lungs into a sterile cup. The sample is taken to a labarotory and placed in a medium under conditions that allow the organisms to grow. A positive culture may identify disease-producing organisms that may help diagnose bronchitis, tuberculosis, a lung abscess, or pneumonia.

        Sputum test

        illustration

      Tests for Sputum stain for mycobacteria

       

      Review Date: 11/14/2016

      Reviewed By: Jatin M. Vyas, MD, PhD, Assistant Professor in Medicine, Harvard Medical School; Assistant in Medicine, Division of Infectious Disease, Department of Medicine, Massachusetts General Hospital, Boston, MA. Also reviewed by David Zieve, MD, MHA, Medical Director, Brenda Conaway, Editorial Director, and the A.D.A.M. Editorial team.

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